Aktivitas Antioksidan dan Antimikrobial dari Ekstrak Plectranthus amboinicus

Abstract

Plectranthus amboinicus or bangun-bangun is a traditional medicinal plant used for postpartum recovery in North Sumatera. The aims of
this study were to detemine antioxidant and antimicrobial activity of leaves Plectranthus amboinicus. The leaves of Plectranthus amboinicus
were extracted with using n-hexane, ethyl acetate and methanol, successively. The extracts were tested to antioxidant activity assay using
DPPH with two fold dillution tehnique and ascorbic acid was used as standard. The antimicrobial activity was conducted using agar diffusion
method against Escherichia coli, Staphylococcus aureus and Candida albicans. The Amoxsan used as positive control for antibacterial
assay, while ketokenazole was for antifungi test. Extracted solvents were used as negative control. The n-hexane and ethyl acetate extracts
did not show any antioxidant activity. While, the IC50 free radical DPPH of methanol extract was 90.96 mg/mL and ascorbic acid was 58.792
mg/mL. The antimicrobial activity of n-hexane and Amoxsan againts Escherichia coli were 11.95 and 7.53 mm. The inhibition zone againts
Staphylococcus aureus were 11.30 and 11.57 mm. The antimicrobial activity of ethyl acetate and Amoxsan againts Escherichia coli were
12.29 and 7.71 mm. The inhibition zone against Staphylococcus aureus were 13.72 and 11.57 mm. The n-hexane and ethyl acetate did not
show any antifungi activity towards Candida albicans. The methanol extract did not show any inhibition zone againts the selected
pathogens.